Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Babcock_Hall on October 26, 2019, 04:36:22 PM
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I poured some polyacrylamide gels last week that turned out extremely sticky and more of a liquid than a solid. Although I was able to run a normal looking pre-stained standard lane, it was almost impossible to pull the plates apart, and there was nothing that I could stain. The gel was more of a goo at this point. I made up fresh acrylamide with a different manufacturer of bis-acrylamide, and the gel looked much more normal prior to running (I am running it right now). Therefore the problem was either the bis, or I simply made a gross error in preparation. Has anyone ever seen a similar result? If not can anyone guess what the problem was? Thanks.