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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: NuzakFarnas on November 01, 2019, 08:29:55 AM

Title: How to quantify the amount of a specific protein in a sample of cells?
Post by: NuzakFarnas on November 01, 2019, 08:29:55 AM

I'm interested in techniques that would allow you to quantify the amount of a specific protein in a cell culture, resulting in the weight (amount) of the protein in each culture sample. I'm aware of ChIP, affinity chromatography, and western blots, but not unaware of a technique to get the total quantity of the protein produced by a cell culture.

Title: Re: How to quantify the amount of a specific protein in a sample of cells?
Post by: Yggdrasil on November 03, 2019, 11:54:23 AM

One way would to be to perform a Western blot alongside a standard curve produced from known amounts of the purified protein.