Chemical Forums
Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: 08roselx on December 02, 2019, 12:06:31 PM
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Hi
I am trying to calculate the SPF of sunscreens using the Mansur equation. I am using a method directly from a paper.
The solutions are made up in ethanol and the UV-Vis is blanked with the ethanol prior to analysis.
I weigh 0.25g sunscreen and make up to 50ml then take 1ml and make to 25ml.
I took a spectrum of one formulation (made in triplicate) and I get breaks in the spectra where there is no absorption value?
I diluted the sample further as at some wavelengths I was getting absorption values of 4 and still the same issue. I also got negative absorption values.
Can anyone help as to what this may be as I have never seen this before?
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Can you show a sample spectrum? An OD of 4 is way too high. You should try to be below 1 if possible.
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I think your detector might be saturated because the concentration is way too high. You mean Absorbance values, not OD right?
I tend not to trust absorbance values over 2.
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The solvent that I had been using was contaminated which is why further dilution didn't help me. Ran samples again today using a different batch of solvent and all was fine :) I knew the absorbance was too high that's why I diluted the samples further (even though I knew it shouldn't have been high as I was following a standard protocol) but dilution didn't reduce the abs values as it was contaminated with some other substance.