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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: txxplc110 on May 08, 2020, 07:53:35 AM

Title: HPLC: Can I read peak resolution from two different chromatograms?
Post by: txxplc110 on May 08, 2020, 07:53:35 AM

Hello!
I have a question about HPLC chromatograms. I got two chromatograms, each one with different signal/wavelength (290nm and 320nm) and I got two peaks of compounds that I wanted to analyze, but the peaks are not on the same chromatogram. My question is, can I read peak resolution of two peaks from two different chromatograms, or not? It doesn’t make any sense for me, but it's in my instruction. I can’t find any information about that. I would be most grateful if you would look into this matter.

Title: Re: HPLC: Can I read peak resolution from two different chromatograms?
Post by: shchavel on May 09, 2020, 03:23:45 AM

Hello!
I am not a HPLS specialist, but i think, that it's uncorrect. You must get this peaks in 1 expirement/chromatogramm to correct compare.

Title: Re: HPLC: Can I read peak resolution from two different chromatograms?
Post by: txxplc110 on May 09, 2020, 02:51:07 PM

Quote from: shchavel on May 09, 2020, 03:23:45 AM

Hello!
I am not a HPLS specialist, but i think, that it's uncorrect. You must get this peaks in 1 expirement/chromatogramm to correct compare.

It turned out to be correct. It was in the same injection but at a different wavelength and an expert told me that I can calculate it. :)