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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: txxplc110 on May 09, 2020, 02:43:08 PM

Title: HPLC column efficiency
Post by: txxplc110 on May 09, 2020, 02:43:08 PM
Hello!
I have a question about HPLC. My peak is very small (it’s more like a little line). It is definitely not like Gauss peak, but I have to find column efficiency. Can I calculate it using the formula Ne=16*(t’r/Wb)^2? Or what else should I do? I found the information that I can only calculate it when the peak is big.