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Chemistry Forums for Students => Undergraduate General Chemistry Forum => Topic started by: Starstreak on September 28, 2020, 08:15:57 PM

Title: Zeroing a cuvette in a Spec
Post by: Starstreak on September 28, 2020, 08:15:57 PM

Hey everyone,

I have a quick question about cuvettes.

So I know you have to zero the cuvette with a blank so that the spectrophotometer does not measure the absorbance of the glass, and then you would want to ideally use the same cuvette for the experiment from that point on.

If for some reason you can't use that cuvette anymore (say you drop it and it breaks) is it okay to switch cuvettes, provided that you re-zero your spectrophotometer to the new cuvette?
I would say yes it is okay based on my understanding of zeroing, but I wanted to double-check on this.

Edit: Also, this is not a homework question, I'm only posting under "undergrad" since I'm not a real chemist so I didn't want to infringe on the other categories.

Title: Re: Zeroing a cuvette in a Spec
Post by: chenbeier on September 29, 2020, 02:03:37 AM

If you set the new cuvette to zero then its ok.