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Chemistry Forums for Students => High School Chemistry Forum => Topic started by: crustd on December 26, 2020, 03:04:03 PM

Title: Would Iron(III) Chloride react with tyrosine to induce a colour change?
Post by: crustd on December 26, 2020, 03:04:03 PM

I'm trying to do a protease assay, but my school doesn't have Phenol reagant. Is it possible to use Iron(III) chloride to react with the tyrosine. Would there be a noticeable colour change that is quantifiable without a spectrophotometer? To my knowledge, tyrosine has a phenol group and I read that Iron(III) chloride would react with phenol groups. Also, what concentration of Iron(III) Chloride solution would be adequate and in what amount?

Title: Re: Would Iron(III) Chloride react with tyrosine to induce a colour change?
Post by: chenbeier on December 26, 2020, 04:00:00 PM

I would say Yes. The best is try and error.