Chemical Forums
Chemistry Forums for Students => High School Chemistry Forum => Topic started by: scienceaddiction1 on May 26, 2021, 04:05:52 PM
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I am trying to measure the absorbance of samples which have varying levels of emulsified bisphenol-a in them with a spectrophotometer. I'm using the absorbance vs concentration setting and am also using a sample that does not have bisphenol-a as a calibrant.
However, when I go to measure the samples (same quantity and procedure) I get extremely small absorbance level differences (.001-.003) which are within the margin of error. I've tried this at 230 and 270nm wavelengths, which are said to be optimal for bisphenol-a detection.
The amount of BPA that should be in these samples is non-negligible and is high compared to real world scenarios.
What could I be doing wrong?
Thank you for any that can be offered!
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Does the small absorbance relates only to samples of smallest concentration or also every other sample ?
The amount of BPA that should be in these samples is non-negligible and is high compared to real world scenarios
Are these samples a field samples (taken to measure concentration of bis-phenol) or are they calibration samples ?