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Chemistry Forums for Students => Organic Chemistry Forum => Topic started by: ICEcream-adikt on June 11, 2021, 10:10:07 AM

Title: Amberlite is "melting"???
Post by: ICEcream-adikt on June 11, 2021, 10:10:07 AM
Hello all, I am a graduate student who's currently working in protein isolation from biomass.

I have protein sources that are dissolved at 10% (w/v) in pH 9, whose supernatant from centrifugation is subjected to overnight amberlite immersion (10% w/w) to remove antinutrient polyphenols.  The problem is that it sometimes breaks down into such very fine powde to the point it almost looks like it melted in the solution. It settles on the bottom over an hour or so, and while I may be able to sieve it out with a very fine mesh, I obviously can't use this. It also happens to some samples but not the other. What could have caused this "melting" amberlite?
Title: Re: Amberlite is "melting"???
Post by: rolnor on June 13, 2021, 01:16:42 PM
I know that amberlite can degrade in time, is your bottle very old?