Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Babcock_Hall on December 03, 2021, 02:07:24 PM
-
Our interest is in LC/MS of the peptides, and we plan to work in water/acetonitrile/formic acid. We found a number of protocols for Zip Tips (ours have C-18 packing) on-line, and we are focusing on those protocols that specify formic acid (not TFA). I can post links to anything that we found. (1) Are Zip Tips necessary? (2) Given their capacity, does one obtain enough for material for one or for several LC/MS runs? (3) Are Zip Tips reusable? (4) Does anyone have a particular paper or protocol that is particularly informative? Thank you very much.