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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Soulja on December 21, 2021, 06:42:14 PM

Title: Analyzing protein sample via UV-Vis
Post by: Soulja on December 21, 2021, 06:42:14 PM

Hey all,

I am working on a project involving an extraction of an allergen protein. I was able to extract it from the raw source, and it is currently in a freezer. My work requires me to analyze it via several methods of analysis. I am having trouble running my protein via UV-Vis; the main problem being that my protein just doesn't seem to be soluble in any solvents I use. So far I've tried acetone and methanol (which was used in the extraction process), but i've had no luck with sonication and stirring. Does anyone have any potential suggestions?

THanks.

Title: Re: Analyzing protein sample via UV-Vis
Post by: jeffmoonchop on December 22, 2021, 03:04:32 PM

How about water?

Title: Re: Analyzing protein sample via UV-Vis
Post by: Orcio_87 on December 22, 2021, 04:23:15 PM

Water may not help if the methanol denatured the protein.

Title: Re: Analyzing protein sample via UV-Vis
Post by: Corribus on December 23, 2021, 01:40:17 PM

What are you hoping that UV-Vis will tell you?

Title: Analyzing protein sample via UV-Vis
Post by: Soulja on December 25, 2021, 03:44:42 PM

Hey all,

I am working on a project involving an extraction of an allergen protein. I was able to extract it from the raw source, and it is currently in a freezer. My work requires me to analyze it via several methods of analysis. I am having trouble running my protein via UV-Vis; the main problem being that my protein just doesn't seem to be soluble in any solvents I use. So far I've tried acetone and methanol (which was used in the extraction process), but i've had no luck with sonication and stirring. Does anyone have any potential suggestions?

THanks.

Title: Re: Analyzing protein sample via UV-Vis
Post by: billnotgatez on December 25, 2021, 04:54:36 PM

we do not double post here
check forum rules
I merged past and present posts
This is a forum policy.
Click on the link near the top center of the forum page.
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http://www.chemicalforums.com/index.php?topic=65859.0

Title: Re: Analyzing protein sample via UV-Vis
Post by: Babcock_Hall on December 30, 2021, 10:56:15 AM

Buffered water is the first thing that I can think of.  Acetone is likely to precipitate a protein.

Title: Re: Analyzing protein sample via UV-Vis
Post by: morphism on January 07, 2022, 08:49:46 AM

Hi! What kind of allergen protein?