Chemical Forums

Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: electriclizard69 on September 25, 2023, 03:44:52 AM

Title: Which wavelength should I consider when dealing with maximum absorbance of a dye
Post by: electriclizard69 on September 25, 2023, 03:44:52 AM

I wish to plot the wavelength of the maximum absorbance of a dye against another quantity that I will be measuring in an experiment. However, certain dyes, such as N719, N3, etc. have multiple peaks in their absorption spectra. Which wavelength should I consider in that case?

For example this absorption spectra for N719: https://www.researchgate.net/figure/Absorbance-spectrum-of-N719-and-BD-dye-solution-in-ethanol-10-10-5-M_fig1_302449760, which wavelength should I consider?

Title: Re: Which wavelength should I consider when dealing with maximum absorbance of a dye
Post by: Borek on September 25, 2023, 05:21:07 AM

There is no simple rule, a lot depends on the circumstances. Given several wavelength to choose from I would select one that sits in the middle of the instrument range and/or one that has as low as possible interference from other substances possibly present in the samples.