Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Snake on February 17, 2007, 05:23:12 AM
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hey guys, does anyone know what will happen if i prepared the agarose gel with water instead of using the buffer to make it?
the agarose gel is used to in electrophorsis to separate the DNA.
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Yeah, when you run the gel it will fal apart.
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The ions in the buffer allow the gel to conduct electricity.
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Is there a biological / scientific question that you would like to answer by not using the normal electrophoresis buffer? The reason I ask, is that it might be that you could get the question answered by using a different buffer / electrophoretic conditions / different separation method.
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what will happen if i prepared the agarose gel with water instead of using the buffer to make it?
Is there a biological / scientific question that you would like to answer by not using the normal electrophoresis buffer? The reason I ask, is that it might be that you could get the question answered by using a different buffer / electrophoretic conditions / different separation method.
Gels made in water will solidify clearer than gels made with buffer
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When I was talking about answering a scientific question, I meant a scientific question regarding the DNA rather than the gel. :)
... and the specific question that came to mind related to the 'strandness' of the DNA, i.e. is it single or double-stranded...
See: http://www.natureprotocols.com/2007/01/25/twodimensional_strandnessdepen.php for an example.
I realise that it is probably a sidetrack from the original question, but thought there might have had a reason for wanting to try different separation conditions.