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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: MNE on August 10, 2007, 04:48:15 PM

Title: Sample preparation for Chromium quantification in algae cell
Post by: MNE on August 10, 2007, 04:48:15 PM

I´m a limnologist, and I´m working with a Selenastrum (algae) in a bioassy under Chromium contamination. I`m asking for help: Wich is the best digestion program for decomposing algae cells, and wich is the best analytical method to quantify the concentration of the chromium accumulated in the Selenastrum cells.

Title: Re: Sample preparation for Chromium quantification in algae cell
Post by: Mr Peanut on August 15, 2007, 05:01:36 PM

US EPA's SW-846 has a method for sediment that will adapt well. Grinf the sample in a tissue grinder first.

http://www.epa.gov/sw-846/main.htm

Use inductively coupled argon plasma atomic emission spectroscopy for the determinative step. If you use AA you'll have some matrix troubles. Also, the absorption vs conc function is curved if the flame isn't carefully adjusted.