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Chemistry Forums for Students => Undergraduate General Chemistry Forum => Topic started by: seanifred on October 10, 2007, 11:01:42 PM

Title: Standard curve for albumin
Post by: seanifred on October 10, 2007, 11:01:42 PM

So I  have a question on my biochem lab that shows the me standard curve (spectrophotometry) for albumin at 540 nm.  The actual question is for me to sketch the curve obtained for the same sample that has absorbance measured at 600 nm.  How do these relate (I think it has to do with the molar absorptivity but I don't know!!)

Thank you  :)

Title: Re: Standard curve for albumin
Post by: ARGOS++ on October 11, 2007, 03:37:04 PM

Dear Seanifred,

For the identical sample in UV/Vis spectroscopy you can use the “Special Beer-Lambert Law” and set for both wavelength:
 

A_λ1 = ε_λ1 * c * d   AND:   A_λ2 = ε_λ2 * c * d   

 
As for the identical Sample c and d in both cases are identical you will end in
 

ε_λ2 = ε_λ1 * A_λ2 / A_λ1

I hope it may be of help.

Good Luck!
                    ARGOS⁺⁺

P.S.: I always thought that albumin is derived from “white”, what should introduce, that in the range 500 nm till 700 nm you should NOT find any Absorption!
.