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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: billitar on February 01, 2005, 07:40:23 PM

Title: calculating pka from absorbance
Post by: billitar on February 01, 2005, 07:40:23 PM

im trying to calculate the pka of  based on experimental data in the form of absorbance at 450nm. the absorbance naturally changes with pH.

I have been trying to manipulate Beer's Law and the H-H buffer equation to awnser this question, but to no avail.

Title: Re:calculating pka from absorbance
Post by: Tetrahedrite on February 01, 2005, 11:29:06 PM

Beer's law only hold true between specific concentrations (ie absorbances). Maybe you're outside the range?