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Specialty Chemistry Forums => Chemical Engineering Forum => Topic started by: dionl on April 30, 2008, 10:29:07 AM

Title: e.coli test
Post by: dionl on April 30, 2008, 10:29:07 AM

Hi, are there anybody who knows how can I prepare a test to carry out the total e.coli form?
I know sodium thiosulfate solution is used , but I don't know the amount of sodium thiosulfate :-\

Title: Re: e.coli test
Post by: eugenedakin on April 30, 2008, 07:54:24 PM

Hello dionl,

Sorry, I am having a difficult time understanding your question:

Quote

are there anybody who knows how can I prepare a test to carry out the total e.coli form?

What are you trying to accomplish: grow cells? Identify type? Determine amount? Is this in wastewaster?

Thanks for your help.

Sincerely,

Eugene

Title: Re: e.coli test
Post by: Gerard on April 30, 2008, 09:46:11 PM

tip read: standard test methods for water and wasterwater methods....
but there are different methods for doing that...
culturing needs agar....
if you want to determine your water is contaminated you can use the plate counting methods or the color reaction method
or you can buy a water testing kit that is sold at you local water testing agency

Title: Re: e.coli test
Post by: dionl on May 06, 2008, 03:03:28 AM

Quote from: eugenedakin on April 30, 2008, 07:54:24 PM

Hello dionl,

Sorry, I am having a difficult time understanding your question:

Quote

are there anybody who knows how can I prepare a test to carry out the total e.coli form?

What are you trying to accomplish: grow cells? Identify type? Determine amount? Is this in wastewaster?

Thanks for your help.

Sincerely,


Eugene

Hi Eugene, I am trying to prepare a coli test to carry out coli form from the water? so I am trying a test for water ;) but I am not sure about the amounts of sodium thiosulfate in solution ? ???not growing :-\

Title: Re: e.coli test
Post by: eugenedakin on May 06, 2008, 08:09:26 PM

Hi dionl,

Artificial student is quite right.  The Standard Methods for water and wastewater have these approved tests.  Be careful when performing these tests on your own, as there are legal implications to be considered.

I hope this points you in the right direction.

Sincerely,

Eugene

Title: Re: e.coli test
Post by: dionl on May 07, 2008, 04:33:33 AM

Quote from: eugenedakin on May 06, 2008, 08:09:26 PM

Hi dionl,

Artificial student is quite right.  The Standard Methods for water and wastewater have these approved tests.  Be careful when performing these tests on your own, as there are legal implications to be considered.

I hope this points you in the right direction.

Sincerely,

Eugene

thank you I understand.. instead of preparing I will find it  ::)

Title: Re: e.coli test
Post by: Gerard on May 11, 2008, 02:05:41 AM

ill give you a non-approved way of testing if your water is contaminated...
first put a small amount of table sugar in your water sample..
incubate for a day at 35 degress celsius and aerate the water,afterwards check for turbidity, if your water is turbid try dropping a 500 mg of powdered penicillin into your water if the turbidity faeds then it is bacteria infested..