Chemical Forums

Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: susanmcqueen on May 02, 2008, 12:13:14 PM

Title: immunoassays
Post by: susanmcqueen on May 02, 2008, 12:13:14 PM

Does anybody know how immunoassays are used in medicinal chemistry?

Title: Re: immunoassays
Post by: JGK on May 05, 2008, 09:39:28 AM

I think you will find a host of information on Google

Title: Re: immunoassays
Post by: AhmedEzatAlzawalaty on May 07, 2008, 09:48:34 PM

i wouldnt assume that immunoanalysis is possible in medicinal chemistry since immunoassay is in fact dependent on antigen-antibody reaction , and since most drugs are relatively small molecular weight with respect to antigen and not that complex in structure as antigen is so they have very low probability to react with antibody.They are haptens not antigens . if u could find a protein for each drug to bind with, it can react with antibody. That is what i think.

Title: Re: immunoassays
Post by: JGK on May 09, 2008, 03:01:33 PM

However, you are ignoring those drugs which are small proteins and not small molecules  and may act as antigens. 

Title: Re: immunoassays
Post by: AhmedEzatAlzawalaty on May 11, 2008, 10:46:34 AM

YES THATS RIGHT

Title: Re: immunoassays
Post by: AhmedEzatAlzawalaty on May 11, 2008, 10:49:09 AM

YOU CAN USE ENZYME-LINKED IMMUNOSORBENT ASSAY OR FLOURESCENT IMMUNO ASSAY OR IMMUNOPRECIPTATION FOR DRUGS WHIC ARE SMALL PROTEINS OR PEPTIDES LIKE INSULIN.DRUGS AS HEPARIN FOR WHICH IMMUNE RESPONSE CAN BE TRIGGERED MAY BE ASSAYED BY ELISA , IF OR IP.

Title: Re: immunoassays
Post by: Borek on May 11, 2008, 11:43:06 AM

Stop abusing capital letters.

Title: Re: immunoassays
Post by: Gerard on May 12, 2008, 11:04:28 AM

immunoassays work like this:
an antibody is attached to a assay plate a small amount of sample, like blood is placed into the plate and washed with distilled water then after a a color reaction procedure is next, a color reagaent is placed on the washed sample then washed again with distilled water if the color retains then the sample is positive,if not then it is negative....
medecinal chemistry?uhm i think what your trying to say is diagnostic chemistry not medecinal chemistry...