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Chemistry Forums for Students => Physical Chemistry Forum => Topic started by: vera on September 06, 2008, 03:48:06 PM

Title: fluorescence spectra
Post by: vera on September 06, 2008, 03:48:06 PM

Emission spectra of tryptophan (Trp) in heated and non-heated model system was recorded at lambda (em) = 300-400 nm, lambda (ex) = 290 nm; and maximum intensity was observed at 340 nm.

Then excitation spectra was recorded at lambda (ex) = 250-320 nm, lambda (em) = 340 nm; and maximum intensity was observed at 280 nm.

We can see lower intensity in emission and excitation spectra in heated model system.

But the question is:
What else we can see from described emission and excitation spectra of Trp ? What kind of information ?

Thanks in advance !