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Chemistry Forums for Students => Organic Chemistry Forum => Topic started by: hal68k on September 09, 2009, 03:44:03 PM

Title: Lipid removal while leaving lipid solute - Prep for LC-MS/MS
Post by: hal68k on September 09, 2009, 03:44:03 PM

Hello all, I am in desperate need of some assistance, right now I am working on detecting pesticides and their metabolites (Deltamethrin, 3-Phenoxybenzoic Acid, etc...) and need some suggestions for removing lipids without removing the pyrethroids, any suggestions would be greatly appreciated! all our samples are prepped in HPLC grade Methanol using a solid-phase extraction technique if that makes a difference.

Title: Re: Lipid removal while leaving lipid solute - Prep for LC-MS/MS
Post by: Don Shelly on September 10, 2009, 02:14:15 PM

Hi,

Lipids should not be soluble in methanol.  You can try putting your extract in a freezer and removing the solidified lipids.  I will attempt to attach a method for extracting pesticides from high lipid matrices.

Don

Title: Re: Lipid removal while leaving lipid solute - Prep for LC-MS/MS
Post by: hal68k on September 16, 2009, 02:26:13 PM

Thank you for your reply, I used a vacuum chamber to evaporate the methanol, then re-constituted the solution with methanol and ran it through a .2 micron filter and it worked beautifully