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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: meme on June 14, 2005, 01:46:48 PM

Title: GFP protein
Post by: meme on June 14, 2005, 01:46:48 PM
Hi everyone,

I'm having some trouble finding information about purifying rGFP using an Ni+2 agarose column. We just did a lab where we had to purify rGFP. I don't have a biochem textbook, and I wasn't able to listen to the lecture about this lab. I tried searching online for more information about it, like about the binding to the 6-his tag and about the protocol itself, but I'm not having luck finding anything. If you do know of a site which can help me, please post a link.. Because of not having a full understanding about this, I'm having trouble answering some questions in my lab report like:

Any help would be greatly appreciated - thanks so much! :)