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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: naj4279 on October 11, 2009, 12:04:26 PM

Title: enzyme kinetic
Post by: naj4279 on October 11, 2009, 12:04:26 PM

Hi,

I run an enzyme kinetic assay and plotted the LB curve. My y-axis is 1/v in (absorbance/time) unit. How do i change this into ug to give a reasonable Vmax value?

Title: Re: enzyme kinetic
Post by: Yggdrasil on October 11, 2009, 06:09:31 PM

You need to know the extinction coefficient of the substance whose absorbance you are measuring.  Try constructing a standard curve to determine this value.