Chemical Forums

Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: 8888kirbz on November 23, 2009, 07:07:28 PM

Title: proper staining technique.
Post by: 8888kirbz on November 23, 2009, 07:07:28 PM

What is the proper procedure in staining blood smears with acridine orange?

Title: Re: proper staining technique.
Post by: JGK on November 24, 2009, 12:10:04 PM

Check the bottom of Page one [url][http://www.pmlmicro.com/assets/TDS/885.pdf/url]

Title: Re: proper staining technique.
Post by: 8888kirbz on November 27, 2009, 08:18:31 AM

that's exactly what i've done sir... but some of my slides having blood smears has its cells not so clearly stained. I dont know what went wrong. Will it be ok if i'll jus restain those slides?

Title: Re: proper staining technique.
Post by: JGK on November 27, 2009, 03:40:51 PM

To be honest I wouldn't know the last time I satined blood smears was in the 1980s (using H&E stain).

However, I wouldn't recommend restaining all of the slides at once. Try one or two first to see if they will take more stain.