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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: asqa on July 06, 2005, 02:31:34 AM

Title: Why?What?How?
Post by: asqa on July 06, 2005, 02:31:34 AM
If we take a 1:1 weight ratio of two solvent and it is analysed by GC then we will not get 50:50? -Reason

Same things holds good in HPLC also.Why?

Please Substantiate?
Title: Re:Why?What?How?
Post by: DrCMS on July 06, 2005, 03:56:05 AM
If you analyse them and just look at area% then you won't get 50:50 due to the varying reponse of the two solvents in FID or UV detection.
Title: Re:Why?What?How?
Post by: asqa on July 06, 2005, 04:23:32 AM
Dr. CMS If we take total max plot instead of taking absorbance at wavelength maxima in uv whether we may get 50:50 ?
Title: Re:Why?What?How?
Post by: DrCMS on July 06, 2005, 10:46:23 AM
That may be better but your two solvents will not have identical total absorbances.  You'd need to calibrate with standards to get the correct answer.