Chemical Forums

Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Jaomski on May 14, 2010, 01:45:39 PM

Title: Protein extraction
Post by: Jaomski on May 14, 2010, 01:45:39 PM

How do you extract proteins from mithocondria and the nucleus respectively...General procedure...thx :)

Title: Re: Protein extraction
Post by: JGK on May 28, 2010, 12:42:55 PM

http://en.wikipedia.org/wiki/Cell_fractionation (http://en.wikipedia.org/wiki/Cell_fractionation)

Title: Re: Protein extraction
Post by: JGK on June 04, 2010, 01:08:23 PM

Try reading it and following the cell disruption link

Title: Re: Protein extraction
Post by: Yggdrasil on June 04, 2010, 02:08:38 PM

Current Protocols in Cell Biology (http://www.mrw.interscience.wiley.com/emrw/9780471143031/cp/cpcb/toc) would be a good place to look.  For example, chapter 3 is on subcellular fractionation and isolation of organelles.