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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: lufthoved on August 11, 2005, 06:35:09 AM

Title: DNA gel electrophoresis
Post by: lufthoved on August 11, 2005, 06:35:09 AM

Hi  everyone...

Im working with DNA and PCR and wanted to test my amplicons. Therefore I made a gel.

Actually it turned out very good but I was wondering how to improve my result.

1. There a large white shadow behinds my band (Im using EtBr and dye). How can I reduce this (or better yet remove it)?

2. Should I run longer time, so the bands will end up post the shadow?

Pardon my english (I come from Denmark)...

Best wishes
lufthoved

Title: Re:DNA gel electrophoresis
Post by: sdekivit on August 11, 2005, 11:56:47 AM

i think you need to run longer

Title: Re:DNA gel electrophoresis
Post by: lufthoved on August 12, 2005, 05:08:32 AM

You think the band will be better then, and the shadow smaller?

Title: Re:DNA gel electrophoresis
Post by: Kira on August 24, 2005, 06:51:35 AM

1.You can try to reduce the amount of ethBr.
2.Sometimes is it enough to clean the photo-table.
3.Running the gel longer makes no sense in my opinion.

best wishes kira

Title: Re:DNA gel electrophoresis
Post by: lufthoved on August 29, 2005, 03:24:13 AM

Hi, all. Thank you for your answers.


I was wondering if it would be better could mix EthBr with the samples and apply to the wells instead of mixing in the gel?