Chemical Forums
Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: ironnica on November 07, 2010, 10:58:01 AM
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I have spent 2 days researching a simple question and cannot find an answer, really need help please.
My experiment involved running IC on a dilute mixture of known samples to create peaks for the anions that eluted. Next i identified peaks by a method known as spiking. (took me until 30 mins ago to find that it is also called co-ingecting {in GC anyway}), raising the conc of one of the solutes in the solution while keeping the others proportional to eachother.
The question in my lab manual is basically this; what are the advantages/disadvantages of peak spiking, and what alternative methods can be used. the latter part is really getting to me.
would really appreciate the help
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Didnt realise that there was so little known about IC
I'm guessing MS could be used so going to have to guess. Thanks to all who looked even.
Closed.
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I usually use a standard (spiked DI) to define retention time. The column generally comes with a certificate that will give you a general idea, as well as order of elution.
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1. a) The advantages/disadvantages of peak spiking is mostly overcoming matrix effects,
b) and what alternative methods can be used - Standard Addition Method (above?)
I recommend that you read textbooks such as Skoog, West ..., + other good references such as online http://en.wikipedia.org/wiki/Standard_addition
www.google.com/search?q=method+of+standard+addition
Cheers!