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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: prince umair on November 23, 2010, 02:05:40 AM

Title: Why does pH affect separation of amino acids by electrophoresis?
Post by: prince umair on November 23, 2010, 02:05:40 AM

Why does the pH affects the separation of amino acids by electrophoresis?
what is the reason?
possible explanation,if given, ill be thankful to all....

and yeah any good reference if provided it will be admired...

Title: Re: Why does pH affect separation of amino acids by electrophoresis?
Post by: Borek on November 23, 2010, 02:58:29 AM

How does electrophoresis work?

What do you know about amino acids in different pH?

Title: Re: Why does pH affect separation of amino acids by electrophoresis?
Post by: rjb on November 23, 2010, 04:18:48 AM

Prince Umair,

Its probably worth refamiliarising yourself with amino acid structure and in particular how the carboxylic acid and amine groups act with varying pH. Have a look at the following link and this should give a nice explanation of amino acid behaviour and should help you answer your question.

http://www.chemguide.co.uk/organicprops/aminoacids/acidbase.html (http://www.chemguide.co.uk/organicprops/aminoacids/acidbase.html)

Kind Regards

R