Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: HB on January 19, 2011, 03:38:04 PM
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I have extracted a polysaccharide from an algae, can it be detected with UV detector or react with any chemical compound so that it can be detected with UV detector or it is impossible
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Sugars have very poor chromophores and are not detected well by UV.
Most chromatography of sugars uses either Refracive Index (RI) or evaporative light scattering (ELS) detection.