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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: stasia222 on September 29, 2005, 04:57:39 PM

Title: disulfide bonds
Post by: stasia222 on September 29, 2005, 04:57:39 PM

Hi, I need to know how or what, other than using Glutathione, can break disulfide bonds at a neutral pH.
Thanks
Stacey

Title: Re:disulfide bonds
Post by: lemonoman on October 01, 2005, 02:56:38 PM

To break the disulfide bond of cystine and form a thiol at each sulfur, they suggest added exces of 2-mercaptoethanol.

See this VERY general mechanism:

(https://www.chemicalforums.com/proxy.php?request=http%3A%2F%2Fimg348.imageshack.us%2Fimg348%2F7017%2Fdisulfide4sw.jpg&hash=5b902d250ffdd22a392001b44afa939691fcc476) (http://imageshack.us)

from http://www.biochemj.org/bj/357/0343/bj3570343.htm (http://www.biochemj.org/bj/357/0343/bj3570343.htm)