Chemical Forums

Chemistry Forums for Students => Organic Chemistry Forum => Topic started by: Moz29386 on October 23, 2011, 04:46:03 PM

Title: Why do we use caffeine as an internal standard in hplc?
Post by: Moz29386 on October 23, 2011, 04:46:03 PM

Hi guys just trying to find out the answer to the above question as I couldnt find it.

Also which compounds are more polar (in order):

Theophyline

Theobromine and

Caffeine?

Thanks!!

Title: Re: Why do we use caffeine as an internal standard in hplc?
Post by: Honclbrif on October 23, 2011, 05:23:33 PM

1) Depends on why you're doing the HPLC

2) Look up the structures