Chemical Forums

Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: calim3ntry on December 06, 2011, 05:53:08 PM

Title: Biotin modifications and reactions
Post by: calim3ntry on December 06, 2011, 05:53:08 PM

Need some advice whether this is even possible. I'm trying to design a biotin that is substituted on one of the N of the ring with a good leaving group. I've seen a bis-N-alkylated (+)-biotin derivative, but I only want one group modified. Then, on the tail, I'd like to have a His, or some functional group containing a His. I then need a good maybe nucleophile to attack aforementioned leaving group so the biotin "head" could be free to then bind to a streptavidin labeled signal. Basically, I need to keep the "head" protected/anchored with the first modification until I introduce the attacking group to then do some kind of substitution reaction to displace it.

Title: Re: Biotin modifications and reactions
Post by: Mitch on December 06, 2011, 06:39:05 PM

Don't spam us with the same topic in multiple forums!

Title: Re: Biotin modifications and reactions
Post by: calim3ntry on December 13, 2011, 11:54:32 AM

Sorry, didn't know. Was looking for help all over.