Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: aghili on November 29, 2012, 01:18:00 AM
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Hi
i have a problem, Please help me :'(
I am a M.Sc. Student in the field of Biotechnology.
I dissolved Sodium butyrate in PBS to use it but Even after several hours is not dissolved. also I've done sonication to dissolve it .
product information of sodium butyrate:
Product Number B5887
Molecular Formula: C4H7NaO2
Molecular Weight: 110.1
CAS Number: 156-54-7
Preparation Instructions
This product is soluble in water (100 mg/ml), with
sonication as needed, yielding a clear to slightly hazy,colorless solution.
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/b5887pis.Par.0001.File.tmp/b5887pis.pdf
best wishes
aghili
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I dissolved Sodium butyrate in PBS to use it but Even after several hours is not dissolved. also I've done sonication to dissolve it .
How much in how much solvent? What is PBS, pardon my ignorance.
Sonication can help speed things up but it isn't magic. Sonication cannot make the insoluble soluble.
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phosphate buffered saline
Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research. It is a water-based salt solution containing sodium chloride, sodium phosphate, and, in some formulations, potassium chloride and potassium phosphate. The buffer's phosphate groups help to maintain a constant pH.
At Beginning I made a 0.5 Molar stock solution (250 miligram sodium butyrate + 4.545 cc phosphate buffered saline(PBS)). After that did not dissolve , again I made a 0.1 molar stock solution (Previous solutions + 18.160 cc phosphate buffered saline(PBS)) But it was not effective.
Thanks for your time.
Best wishes
aghili
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If possible - use potassium salts in buffer.
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Are you allowed any leeway in warming up the solution a bit or not at all?
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no, not at all
I think that the heat may be harmful for this material because After making the stock solution will be unstable and Should be kept at -20.
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I agree with the suggestion made by AWK on the basis of the common ion effect. If you have to store this solution frozen, then you will have similar issues every time you thaw out an aliquot. It may need to be sonnicated, warmed, or whatever.