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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: nitramyelood on March 10, 2014, 12:01:28 PM

Title: GCMS analysis of Rapramycin
Post by: nitramyelood on March 10, 2014, 12:01:28 PM

Hi All
I am trying to determine rapramycin from a Drug eluting stent.I have placed the stent in DMSO to extract the Rapramycin and injected the sample underivatised onto the GCMS unfortunately I can see no peaks yet
any advice greatly appreciated
Nitram

Title: Re: GCMS analysis of Rapramycin
Post by: marquis on March 10, 2014, 12:09:56 PM

Can you give more information?

What is the temperature profile you are using on the GC/MS?
What column are you using? What model GC/MS, what detector,
what flow and carrier gas?

What chemical information can you give on the rapramycin?

Thanks.

Title: Re: GCMS analysis of Rapramycin
Post by: MOTOBALL on March 10, 2014, 03:50:51 PM

I cant find rapramycin; do you mean "rapamycin".

If you do, I'm surprised that you expect a compound with 5x >C=O, 2x -OH groups and MW 914 (underivatized) to have sufficient volatility to be amenable to GC analysis.

Have you done a lit. search, or any homework, on this compound ?

You should be doing HPLC; many refs. out there.