Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Kikko on March 18, 2014, 05:41:35 AM
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The maturation of a flurorophore is a complete chemical system if I understand it correct?
Or are there any enzymes involved in this maturation process?
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Insufficient context for a meaningful reply.
You're going to have to define maturation of a fluorophore., for starters. Because that's a term I don't understand. What the fluorophore in question actually is, and what biochemical molecule its a part of, will also help..
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Insufficient context for a meaningful reply.
You're going to have to define maturation of a fluorophore., for starters. Because that's a term I don't understand. What the fluorophore in question actually is, and what biochemical molecule its a part of, will also help..
I mean, for example, GFP (green fluorescent protein).
It matures in the cells, but I wonder if its a pure chemical process or whether their are also enzymes involved? I don't think that there are enzymes involved in it.
Not sure what other term there is... in literature I just find "maturation" of the fluorophore.
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Helpful?
http://www.olympusfluoview.com/java/fpfluorophores/
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Helpful?
http://www.olympusfluoview.com/java/fpfluorophores/
I know this website.... its the best I could find, but it does not really specify if its a complete chemical process or that enzymes are involved. Based on what I read on that website, I think its purely chemical... but not 100% sure.
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The process may be accelerated by the protein itself. "The prolific use of green fluorescent protein and its variants throughout cellular biology relies on the post-translational formation of the chromophore, which proceeds without the need for any additional enzymes or cofactors, except molecular oxygen."
Chem Soc Rev. 2009 Oct;38(10):2865-75. doi: 10.1039/b903641p. Epub 2009 Aug 26.
Green fluorescent protein: structure, folding and chromophore maturation.
Craggs TD.
Curr Opin Struct Biol. 2006 Dec;16(6):714-21. Epub 2006 Oct 24.
Fluorescent proteins: maturation, photochemistry and photophysics.
Remington SJ.
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The process may be accelerated by the protein itself. "The prolific use of green fluorescent protein and its variants throughout cellular biology relies on the post-translational formation of the chromophore, which proceeds without the need for any additional enzymes or cofactors, except molecular oxygen."
Chem Soc Rev. 2009 Oct;38(10):2865-75. doi: 10.1039/b903641p. Epub 2009 Aug 26.
Green fluorescent protein: structure, folding and chromophore maturation.
Craggs TD.
Curr Opin Struct Biol. 2006 Dec;16(6):714-21. Epub 2006 Oct 24.
Fluorescent proteins: maturation, photochemistry and photophysics.
Remington SJ.
Ah, ok thanks.
I remember finding that paper/title but I had no access to it.
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How about this, from one of the guys who actually discovered it:
http://tsienlab.ucsd.edu/Publications/Tsien%201998%20Annu.%20Rev.%20Biochem%20-%20GFP.pdf