Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Lii on September 20, 2014, 06:38:28 PM
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I'll just say it: proteins REALLY confuse me. UniProt confuses me even more.
Okay, so I have a uniprot number for a specific protein. This protein has an important domain, X. I was supposed to find the most closely related members of the X domain family, so I ran a BLAST on the entire protein and it listed a bunch of other proteins and their percent similarities. So I think I did that right... Or maybe I should just BLAST the domain sequence.
Then I'm asked to produce a sequence alignment and identify the sequence signature for the X domain and I have no idea what to do. I notice there is an align tab but it's not clickable.
After this I have to determine which protein has the shortest X domain using molecular weights. Also not sure if they list molecular weights on there or if I have to calculate them somehow.
If anyone has any tips on how to navigate the site I would be very grateful.