Chemical Forums
Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: gingi85 on September 17, 2015, 04:57:34 PM
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Hello Friends,
I am trying to run an HPLC analysis of a modified nucleoside triphosphate. When I run it using 0.1M TEAB/Acetonitrile as my mobile phase, I get two sharp, good-looking peaks (for two diastereomers). When I run the sample using 0.1M TEAA/Acetonitrile, the first peak comes off nicely, but the second one is severely broadened. the pH of both buffer is identical (7.5). What's going on? Suggestions?
I would like to avoid using TEAB, because of its unstable pH over time, but I need to stay with volatile buffers that are compatible with mass spec.