Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Jefke on November 05, 2015, 04:22:32 PM
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Hallo all,
I saw a post about a competitive inhibitor used in a yeast two hybrid system. Now I had to study this system during my biology classes, but never realised it back then.
If you use a yeast two hybrid system they always say you can use 3-AT, the competitive inhibitor for the enzym you produce with your reporter gene (his3), but would it be possible to just use another product that is a competitive inhibitor of another gene/enzym in the same pathway ?
Or is this too difficult/different from using a direct competitive inhibitor?
Or, the other way around, just use a different enzym/gene as a reporter (but still use the same prodcut: 3-AT?
Any ideas?