Chemical Forums

Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: hell0joe on February 22, 2017, 08:06:13 PM

Title: Dansyl chloride reaction
Post by: hell0joe on February 22, 2017, 08:06:13 PM

My textbook states that dansyl chloride reacts with primary amines to form dansylated polypeptides (for the process of protein sequencing).

Would there be a problem if there was a lysine residue in the polypeptide, since dansyl chloride would also react with the amino group of lysine as well?

Title: Re: Dansyl chloride reaction
Post by: AWK on February 23, 2017, 03:09:18 AM

Amino group of lysine is also reactive.

Title: Re: Dansyl chloride reaction
Post by: Babcock_Hall on February 23, 2017, 09:51:47 AM

The same problem shows up in the use of fluorodinitrobenzene.