Chemical Forums
Chemistry Forums for Students => Organic Chemistry Forum => Organic Chemistry Forum for Graduate Students and Professionals => Topic started by: vikram on May 30, 2017, 08:54:04 AM
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I was reading a literature where I came across the sentence "the filtrate was eluted
with 125 mM NaOH solution"
May I know what is the meaning of this? What exactly I have to do with filtrate?
Thanks in advance:)
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You add NaOH on top of the filtrate suck it trough, you can say wash instead of elute, it is the same thing.
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You add NaOH on top of the filtrate suck it trough, you can say wash instead of elute, it is the same thing.
This is not correct.
The filtrate is the liquid that passes through a filter; the solid that remains on the filter is the retentate.
Elution is a term used in chromatography to describe the passage of a compound along (and eventually from) a chromatographic column.
I think that the original poster should provide some additional experimental detail prior to the step in question.
Regards.
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You add NaOH on top of the filtrate suck it trough, you can say wash instead of elute, it is the same thing.
This is not correct.
The filtrate is the liquid that passes through a filter; the solid that remains on the filter is the retentate.
Elution is a term used in chromatography to describe the passage of a compound along (and eventually from) a chromatographic column.
I think that the original poster should provide some additional experimental detail prior to the step in question.
Regards.
Unfortunately, there is no additional information in the paper :-[
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Sorry, I got confused.
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Unfortunately, there is no additional information in the paper :-[
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If this is from a primary source (a scientific journal) there must be more info, otherwise it is meaningless !
Could you give the source reference?
Journal, year, volume, pages, authors, title.
Regards
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If you explained a little about the chemistry going on here it might help... do you expect that the solid or the solution contains your product? It could be that the solid is your product, and that treatment with NaOH deprotonates and dissolves it, so it's washed away as the sodium salt in the filtrate?