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Chemistry Forums for Students => Organic Chemistry Forum => Topic started by: parisa-fateh on November 13, 2017, 04:37:34 AM

Title: TCEP-HCl
Post by: parisa-fateh on November 13, 2017, 04:37:34 AM

It is told that the TCEP can work in wide range of pH to break disulfide bonds. So why is it recommended that it is better to use TCEP in buffer solution with around natural pH?

Title: Re: TCEP-HCl
Post by: Arkcon on November 13, 2017, 06:46:09 AM

For what target?  Natural pH, that means, what exactly?  I have to guess, you mean physiological pH, meaning the target is a bio-molecule.  But you should say that.  And it may completely answer the question for yourself.

Title: Re: TCEP-HCl
Post by: Babcock_Hall on November 13, 2017, 10:18:06 AM

TCEP is said to be somewhat labile in phosphate buffers.  I am not sure of the mechanism.

https://hamptonresearch.com/product_detail.aspx?cid=4&sid=47&pid=47

Title: Re: TCEP-HCl
Post by: phth on November 21, 2017, 11:29:35 PM

What reduces the disulfide and what pKa does it have?