Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: socomplicated on September 02, 2004, 05:48:18 PM
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here is a part of the protocol we did:
a)spin down the chloroplast solution and decant the supernatant. add 4ml water to the pellet.
b) add 10ul proteinase K ( concentration= 20mg/ml) to get a final concentration of 50ug/ml.
-> how do they get 10ul? which formula do they use? i don't think that they use the dilution formula (C1 XV1= C2 X V2) since we don't know the total volume of the solution. it can't be 4ml, because when we add proteinase K the volume of the solution will increase.
c) then add slowly 0.25ml 10% or 0.125ml 20% SDS to get a final concentration of 0.5% SDS.
-> again, how do they come to 0.25ml 10% or 0.125ml 20% SDS to get a final concentration of 0.5% SDS?
i am very stucked, please *delete me*
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c.) When it says % SDS it most likely means volume/volume. For the 10% case add 0.025ml SDS and 0.135ml water. Or scale it up accordingly.
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SDS can be purchased as a solid - if it is - usually % is grams/ml
so grams of sds/ml total solution