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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: Sgriger534 on December 17, 2018, 08:36:08 PM

Title: Question about type of acid used in Riboflavin assay
Post by: Sgriger534 on December 17, 2018, 08:36:08 PM

So I am currently working on updating some analytical procedures where I work and one is a fluorometric riboflavin assay

My question is that the procedure uses 0.2n Hcl to dissolve the sample.  However every other similar procedure I can find (e.g. USP, AOAC) uses 0.2n acetic acid.
Is there any benefit to using one acid over the other?

Title: Re: Question about type of acid used in Riboflavin assay
Post by: Babcock_Hall on December 17, 2018, 10:13:13 PM

I don't know the answer, but there is going to be a large pH difference, and I would at least consider how stable riboflavin is at the lower pH value.

Title: Re: Question about type of acid used in Riboflavin assay
Post by: Sgriger534 on December 17, 2018, 10:22:25 PM

As in the other methods,  the ph is adjusted (although I can't remember the value currently) but it falls within the same range as the methods using acetic acid