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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: Legolasas5 on February 20, 2019, 04:30:15 PM

Title: Optimisation of elution conditions using designed experiments in HPLC
Post by: Legolasas5 on February 20, 2019, 04:30:15 PM

Hello there,
I am trying to optimise HPLC method and I have a problem with 2 peaks that change their selectivity depending on elution conditions. Is it appropriate to have a negative resolution as a response in the DoE table if these peaks switch.
Thank you for your answers.