Chemical Forums
General Forums => Comments for Staff and Comments from Staff => Topic started by: helpmeplsyeet on March 30, 2019, 05:02:22 AM
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I want to conduct an experiment on the oxidation of ethanol to become ethanal but am unsure how to accurately measure it aside from observing a colour change. Is there some sort of quantitative way to notice that the reaction has occurred? The intent is to measure the reaction rate for the reaction to occur, so in order to accurately do so I've been told I need another way to measure it.
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Ethanal is colorless, so you won't see any color change. But you should be easily able to follow concentration changes with IR spectroscopy, oxidation means producing carbonyl, which is pretty characteristic.
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There are colorimetric assays for aldehydes, you can use that to follow concentration by withdrawing little samples and doing a bit of math.
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Aldehyde, like all carbonyl, has a weak uv vis transition at about 280-290 nm that you could use, depending on the concentration. There is a much stronger one at around 190 nm or so but it is harder to use. Solvent cut off, cuvette type, and your instrument hardware are important to probe that far into uv.
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The enzymatic oxidation is easy to follow in a continuous spectrophotometric assay. However, this may not be something in which you are interested.