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##### Analytical Chemistry Forum / Chromatography

« Last post by**John55**on

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**Today**at 08:05:53 AMhi ,

i'm desperate,could anyone help me please?

We use an analytical column to optimize the separation and then carry out a preparative separation of two substances. We have a 10 cm long preparative HPLC column with 40 separation steps per cm and an analytical column (also 10 cm) with 400 separation steps per cm. Both columns are packed with the same separating material and therefore have the same retention factors for the two components of our two-component mixture. For the preparative separation, the resolution must be at least 2. To what value do we have to optimize the resolution with the analytical column so that we can then transfer the separation to the preparative column?

i'm desperate,could anyone help me please?

We use an analytical column to optimize the separation and then carry out a preparative separation of two substances. We have a 10 cm long preparative HPLC column with 40 separation steps per cm and an analytical column (also 10 cm) with 400 separation steps per cm. Both columns are packed with the same separating material and therefore have the same retention factors for the two components of our two-component mixture. For the preparative separation, the resolution must be at least 2. To what value do we have to optimize the resolution with the analytical column so that we can then transfer the separation to the preparative column?