Could you provide a little more about your exptl procedure?

It seems you are binding liposomes to the surface first (in a monolayer?), then looking to see whether your protein binds to this monolayer. Your positive control looks right. Is it possible your test protein is disrupting the lipid monolayer - i.e., your monolayer is dissociating from the surface, leading to an initial loss in RU?

With respect to the compound on the far right, I would think about the octet rule.

Dear everyone,

I've got a question regarding the functionalization of polyallylamine. I am not much of a polymer chemist, so please treat me with leniency.
As part of my PhD project I would like to functionalize the amines of polyallylamine (or any similar polymer that consists of a aliphatic backbone and has amines in the side-chains). Specifically I would like to react the amine with an Acylchloride, actually with a Fmoc-chloride derivative I synthesized. The reaction I try to achieve can be depicted as something like: https://imgur.com/a/0tHf73S


What would be a proper synthetic strategy to go about it? I know I can usually react amines with Fmoc-chloride in DCM in the presence of DIPEA as a base. But I can not really apply this procedure here, since the polymer behaves weirdly in DCM, as soon as it touches DCM it becomes a gum-like, insoluble, unusable blop. 
I've got two formulations of the polyallylamine: on the one hand the hydrochloride (which of course is not nucleophilic) as a solid https://www.sigmaaldrich.com/NL/en/product/aldrich/283223 and on the other hand I have a watery solution (which is messing with Acylchlorides) https://www.sigmaaldrich.com/NL/en/product/aldrich/479136

I just naively tried to react the watery polymer solution with sulfonated Fmoc-Cl in water/dioxane with Na2Co3 as a base, but under these conditions the Fmoc-Cl would already start cleaving and causing troubles, also I had no proof the desired reaction actually took place.

Does anyone of you know a good strategy on how to functionalize polyallyamine side-chain amines? I would not need to react every single active site on the polymer, I think it would be sufficient if only like 20-40mol% of the amines would be reacted. But how to achieve that? How to access the active sites of these kind of polymers? I am grateful for any suggestion on how to go about it, as well as for any literature on the topic (I could not find lots of useful literature).

Bests

Quote from: Richeyyy on Yesterday at 01:02:50 PM

I have no idea which one to calculate to get the perfect ratio

Ratio of what?

Percent composition is a property of a compound (or mixture), not of a reaction.

Sorry if I didn't mention, and thanks so much for all your help so far I really appreciate it, and regarding your question, it's actually a mixture, I want to figure out how to calculate perfect ratios for a reaction to happen (so that there would be just the right amount of molecules of each reactant, not too much and not too little, for the reaction to happen without any of the reaction's products being greater than the other), I think those are called stoichiometric ratios, right now I wanna learn how to calculate kno3+sugar perfect ratio, but I can't find out how:/

Yes this is the picture.
White - Hydrogen
Red - Oxygen
Black - Carbon
Blue - Nitrogen
Green - Fluorine