Hi. I am attempting to determine the Kinetic parameters of glucose oxidase, specifically max velocity and MM-Constant. I have already conducted an experiment using a colorimetric assay added to different concentrations of glucose along an electron donor (2'-2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS)), and a spectrometer to determine the absorption (which should be linear to the concentration of the colorimetric assay). I am attempting to compare my derived max velocity and MM-Constant in literature.

I had assumed it would be trivial. It was not. So far, I have exhausted the methods I know to locate information on the max velocity and MM-constant. Searching for academic literature or technical documentation on Google, Google Scholar, IEEE XPlore has yielded me many other elements in glucose oxidase, but not max velocity and MM-Constant. Going through the datasheets of glucose oxidase from suppliers such as Sigma-Aldritch fails to provide me with the numbers. I don't know what other avenues I should explore, so I've come here. 

Mix it with lime and use it as fertilizer. Source for Nitrogen.

I have about 15 pounds of cyanuric acid left from a year ago adjusting my pools chlorine stabilizer levels after a refill. Is there anything fun / useful i can use it for around the house or should i just toss it? I do occasionally use acids like ascorbic, citric, phosphoric, sulfuric, etc for cleaning or plating; so not a stranger to acids, but am not sure about this one...
Thanks ahead

To start, I’m sorry that I can’t add any images - I’m on mobile and can’t figure out how to add one. Basically, my professor wants us to propose a mechanism for reacting diethyl malonate with HONO to form an oxime (so the oxime is on the alpha carbon and the rest of the molecule is the same). The first mechanism I did was:

1. HONO forms the nitrosonium ion (gets protonated by H3O+)
2. The carbonyl on diethyl malonate tautomerizes to the enol
3. The electrons from that double bond attach to the N in nitrosonium, the carbonyl reforms, and the electrons from the N-O+ triple bond go to the O
4. The nitroso O gets protonated
5. H2O takes the H from the alpha carbon, those electrons form the C=N bond, and the electrons in the N=O bond go to the O.

The problem is, I was just using a generic H3O+ because every mechanism I have seen for the formation of nitrosonium uses a stronger acid to protonate HONO. Specifically, I’ve mostly seen NaNO2 and HCl used. But my professor said we’re not allowed to use anything other than HONO - so no stronger acid, and no water, either. So my questions are:

1. Is this even the right track for this mechanism?
2. Does the nitrosonium ion form when just HONO is present (do acids protonate themselves?)

UPDATE:  As it turned out, someone in a previous semester mixed the standard Fe solution wrong, so the concentration isn't 10 mg/L we don't know what it is.