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Topic: HPLC theory!  (Read 4923 times)

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Offline daikiri

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HPLC theory!
« on: March 25, 2007, 03:53:08 PM »
Please, help me with some theory in HPLC!
How the concentration of buffer influence on the retention time of the analyte? In my case when I  change the concentration of KH2PO4 buffer from 0.05M to 0.01M (pH=7.4), the retention time of the analysed substance decrease from 15 min to 18 min. 
I need to explain this fact  ???
I think, that  increasing of buffer's concentration leads to growing of the ionic power of the solution,
but what is the result from this?

Thank you!
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Offline Yggdrasil

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Re: HPLC theory!
« Reply #1 on: March 25, 2007, 06:53:51 PM »
What is your analyte and what type of column are you using?
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Offline AhmedEzatAlzawalaty

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Re: HPLC theory!
« Reply #2 on: March 26, 2007, 11:39:04 AM »
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