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Topic: Best solvent choice  (Read 2106 times)

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Offline Alex koosha

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Best solvent choice
« on: January 19, 2016, 02:55:53 PM »
I want to extract and quantify Lipids inside the starch. Which solvents you recommend for the extraction?

Offline Babcock_Hall

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Re: Best solvent choice
« Reply #1 on: January 19, 2016, 05:48:06 PM »
I am not sure what you mean by inside the starch.  However, a classic solvent for extraction of lipids is chloroform/methanol 2:1:  http://www.cyberlipid.org/extract/extr0005.htm

Offline Alex koosha

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Re: Best solvent choice
« Reply #2 on: January 19, 2016, 06:08:02 PM »
I meant the lipids are inside starch granules. May I ask you if we only use one phase extraction for example only chloroform is it a good idea. Starch is not miscible in organic solvent and wil be precipitated and lipids will  remain inside organic phase and could be separated by using pipet. Do you think this method could work?

And my 2nd question is when we use chloroform/methanol starch could not be dissolved nethier in chloroform nor in methanol so is starch precipitated below two phases?

Offline Babcock_Hall

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Re: Best solvent choice
« Reply #3 on: January 19, 2016, 06:26:10 PM »
I am not sure, but the starch may need to be filtered away from the extraction solvent (just a guess).  Have you tried doing a literature search to see what other people have done?

Offline Alex koosha

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Re: Best solvent choice
« Reply #4 on: January 20, 2016, 01:49:31 AM »
Yes I am doing this at the moment but I wanted to ask if anyone has done the same experiment before  :)

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