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Topic: Glucoside half-life?  (Read 3670 times)

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Offline Cereberus1983

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Glucoside half-life?
« on: May 21, 2007, 03:58:45 AM »
Once a plant glucoside has been extracted using ethanol, how long can it remain in the solution without denaturing, and can it be poured into a different medium, a glass of suger for instance, and the alcohol allowed to evaporate off, without damaging the glucoside? Any help would be appreciated...thanks guys...

Offline AWK

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Re: Glucoside half-life?
« Reply #1 on: May 21, 2007, 04:53:48 AM »
Generally, glucosides are relatively stable in neutral and slightly basic medium
AWK

Offline Cereberus1983

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Re: Glucoside half-life?
« Reply #2 on: May 21, 2007, 06:52:06 AM »
Thanks AWK.....What about prefered temperatures...would i be correct in assuming the lower the better....and what about if the ethanol were allowed to evaporate....and the glucoside left to side on another substance, such as suger, as mentioned...would that render it denatured?

Offline AWK

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Re: Glucoside half-life?
« Reply #3 on: May 21, 2007, 08:21:09 AM »
Do not use term denaturation for glucosides. They can decompose or undergo transglucosylation (exchanging of aglycone in the presence of an alcohol and acid - acid catalysis).
Always, the lower temperature, the better stability of compound. Usually we use RT.
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Offline Yggdrasil

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Re: Glucoside half-life?
« Reply #4 on: May 21, 2007, 01:52:38 PM »
For some synthetic glucosides (e.g. isopropyl-thio-galatoside or o-nitrophenyl-galactoside) we store the compound, either in solid or aqueous form, at -20o to prevent degradation.  However, table sugar is a glucoside, and it is very stable at room temperature.

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